Granzyme B Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA43303

应用：

IHC-P,IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

28 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CCPI; CGL 1; CGL-1; CGL1; CSP B; CSP-B; CSPB; CTLA1; CTSGL1; Endogenous granzyme B; Granzyme B; GZMB; HLP; SECT; C11

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IHC-P 1:100-1:300

IF 1:50-1:200

ELISA 1:10000-1:20000

Immunogen

Information

Gene Name：

GZMB

Protein Name：

Granzyme B

Gene ID：

3002 (Human)

14939 (Mouse)

171528 (Rat)

SwissPro：

P10144 (Human)

P04187 (Mouse)

P18291 (Rat)

Subcellular Location：

Secreted. Cytolytic granule.

Immunogen：

Synthesized peptide derived from human Granzyme B. AA range: 81-130.

Specificity:

Granzyme B Polyclonal Antibody detects endogenous levels of Granzyme B protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-Gramzyme B antibody (AWA43303) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA43303) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of NIH3T3 cells labeling Granzyme B with Rabbit anti-Granzyme B antibody (AWA43303) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Granzyme B antibody (AWA43303) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).