Cleaved PARP1(D214) Rabbit Polyclonal Antibody

货号:
AWA43168
应用:
WB,IF
反应性:
Human,Mouse,Pig
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Pig

    Molecular Wt:

    113 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    ADPRT; ADPRT 1; ADPRT1; pADPRT 1; PARP; PARP 1; PARP1; poly (ADP ribose) polymerase 1; Poly [ADP ribose] polymerase 1; Poly[ADP ribose] synthase 1; PPOL


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF 1:50-1:300



    Immunogen

    Information

    Gene Name:

    PARP1

    Protein Name:

    Poly [ADP-ribose] polymerase 1


    Gene ID:

    142 (Human)  
    11545  (Mouse)

    SwissPro:

    P09874 (Human)  
    Q921K2 (Mouse)


    Subcellular Location:

    Chromosome. Nucleus. Nucleus, nucleolus. Cytoplasm, cytosol. Cytoplasm.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human PARP. AA range: 165-214.


    Specificity:

    Cleaved PARP1 (D214) Polyclonal Antibody detects endogenous levels of fragment of activated PARP1 protein. resulting from cleavage adjacent to D214.




    Product images
    Cleaved PARP1(D214) Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Cleaved PARP(D214) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA43168, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A549 cell
    Lane 2: HUVEC cell
    Lane 3: MCF-7 cell


    Exposure time: 3 min
    Predicted band size: 113 kDa
    Observed band size: 25 kDa
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