HMGB1 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA43122

应用：

IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

25 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

high mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3; HMGB1; SBP 1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IF 1:50-1:200

ELISA 1:20000

Immunogen

Information

Gene Name：

HMGB1

Protein Name：

High mobility group protein B1

Gene ID：

3146 (Human)

15289 (Mouse)

25459 (Rat)

SwissPro：

P09429 (Human)

P63158 (Mouse)

P63159 (Rat)

Subcellular Location：

Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome.

Immunogen：

Synthesized peptide derived from the N-terminal region of human HMGB1.

Specificity:

HMGB1 Polyclonal Antibody detects endogenous levels of HMGB1 protein.

Product images
	Fig: Immunocytochemistry analysis of HELA cells labeling HMGB1 with rabbit anti-HMGB1 antibody (AWA43122) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-HMGB1 antibody (AWA43122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488 AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Immunocytochemistry analysis of Raw264.7 cells labeling HMGB1 with rabbit anti-HMGB1 antibody (AWA43122) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-HMGB1 antibody (AWA43122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488 AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).