CD63 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA43088

应用：

WB,IHC-p,IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

26,35-65 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CD63; CD 63; CD63_HUMAN; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; Granulophysin; ME491; MLA1; MLA 1; OMA81H; Tetraspanin 30; Tetraspanin-30; Tspan 30; Tspan-30; TSPAN30; LAMP-3; LAMP 3; LAMP3; LIMP; NGA; PTLGP40

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-1:300

IF 1:50-1:200

ELISA 1:20000

Immunogen

Information

Gene Name：

CD63

Protein Name：

CD63 antigen

Gene ID：

967 (Human)

12512 (Mouse)

29186 (Rat)

SwissPro：

P08962 (Human)

P41731 (Mouse)

P28648 (Rat)

Subcellular Location：

Cell membrane. Lysosome membrane. Late endosome membrane. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface.

Immunogen：

The antiserum was produced against synthesized peptide derived from the Internal region of human CD63. AA range: 121-170.

Specificity:

CD63 Polyclonal Antibody detects endogenous levels of CD63 protein.

Product images
	Fig: Immunocytochemistry analysis of Hela cells labeling CD63 with Rabbit anti-CD63 antibody （AWA43088）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-CD63 antibody （AWA43088 ）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-CD63 antibody (AWA43088) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA43088) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of CD63 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA43088, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1:Hela cell Lane 2: Rat heart Lane 3: Rat liver