HSP90AB1 Rabbit Polyclonal Antibody

货号:
AWA42994
应用:
WB,IF,IP,ELISA
反应性:
Human,Mouse,Rat,Monkey
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Monkey

    Molecular Wt:

    83 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    HSPC3; HSPC2; HSP90B; Heat shock protein HSP 90-beta; Heat shock protein HSP 90 beta; HSP84; HSPCB; D6S182; Hsp90β

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IF 1:50-1:1000

    IP 2-5 ug/mg lysate

    ELISA 1:40000

     



    Immunogen

    Information

    Gene Name:

    HSP90AB1

    Protein Name:

    Heat shock protein HSP 90-beta


    Gene ID:

    3326 (Human)

    15516 (Mouse)

    301252 (Rat)

    SwissPro:

    P08238 (Human)

    P11499 (Mouse)

    P34058 (Rat)

     


    Subcellular Location:

    Cytoplasm. Melanosome. Nucleus. Secreted. Cell membrane. Dynein axonemal particle. Cell surface.

     


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human HSP90B. AA range: 226-275.

     


    Specificity:

    HSP90AB1 Polyclonal Antibody detects endogenous levels of HSP90AB1 protein.


    Product images
    HSP90AB1 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of HSP90AB1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42994, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HSC-T6 cell
    Lane 2: NIH3T3 cell
    Lane 3: HEK-293cell
    Lane 4: PC-12 cell
    Lane 5: HELAcell
    Lane 6: 4T1 cell
    Lane 7: Jurkat cell
    Lane 8: RBL-2H3 cell
    Lane 9: NPK-49Fcell
    Lane 10: PC-3 cell




    Predicted band size:83 kDa
    Observed band size:90 kDa
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