Collagen I Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA42978

应用：

IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

139 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Alpha 1 type I collagen; COL1A1; Collagen alpha 1(I) chain; Collagen type I alpha 1; EDSC; OI1; OI2; OI3; OI4; type I proalpha 1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IF 1:50-1:300

ELISA 1:5000-1:20000

Immunogen

Information

Gene Name：

COL1A1

Protein Name：

Collagen alpha-1(I) chain

Gene ID：

1277/1278 (Human)

12842/12843 (Mouse)

29393/84352 (Rat)

SwissPro：

P02452/P08123 (Human)

P11087/Q01149 (Mouse)

P02454/P02466 (Rat)

Subcellular Location：

Secreted, extracellular space, extracellular matrix.

Immunogen：

Synthesized peptide derived from Collagen I. AA range: 1200-1217.

Specificity:

Collagen I Polyclonal Antibody detects endogenous levels of Collagen I protein.

Product images

Collagen I Rabbit Polyclonal Antibody - 1

Fig: Immunocytochemistry analysis of PANC-1 cells labeling Collagen I with rabbit anti-Collagen I antibody (AWA42978) at 1/50 dilution (green).

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-COL1A1 antibody (AWA42978) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).