SP1 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA42957

应用：

IHC-P,IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

81 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

SP1; SP 1; SP1_HUMAN; Sp1 transcription factor; Transcription factor Sp1; Specificity protein 1; TSFP1; TSFP 1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IHC-P 1:100-1:300

IF 1:50-1:300

ELISA 1:20000

Immunogen

Information

Gene Name：

SP1

Protein Name：

Transcription factor Sp1

Gene ID：

6667 (Human)

20683 (Mouse)

24790 (Rat)

SwissPro：

P08047 (Human)

O89090 (Mouse)

Q01714 (Rat)

Subcellular Location：

Nucleus. Cytoplasm.

Immunogen：

The antiserum was produced against synthesized peptide derived from human SP1. AA range: 706-755.

Specificity:

SP1 Polyclonal Antibody detects endogenous levels of SP1 protein.

Product images

Fig: Immunocytochemistry analysis of U2OS cells labeling SP1 with Rabbit anti-SP1 antibody (AWA42957) at 1/50 dilution(green ).

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SP1 antibody (AWA42957) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).