FABP1 Rabbit Polyclonal Antibody

货号:
AWA42859
应用:
WB,ELISA
反应性:
Human,Mouse,Rat,Zebrafish
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Zebrafish 

    Molecular Wt:

    14 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    FABP1; FABPL; Fatty acid binding protein 1; L FABP; liver FABP


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:2000

    ELISA 1:5000-1:20000



    Immunogen

    Information

    Gene Name:

    FABP1

    Protein Name:

    Fatty acid-binding protein, liver


    Gene ID:

    2168 (Human)     

    14080 (Mouse)     

    24360 (Rat)

     

    SwissPro:

    P07148 (Human)     

    P12710 (Mouse)     

    P02692 (Rat)

     


    Subcellular Location:

    Cytoplasm.


    Immunogen:

    Synthesized peptide derived from human FABP1.


    Specificity:

    FABP1 Polyclonal Antibody detects endogenous levels of FABP1 protein.




    Product images
    FABP1 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of FABP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42859, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: HEP3B2.1-7 cell lysate
    Lane 4: SK-HEP-1 cell lysate
    Lane 5: BEL7402 cell lysate

    Predicted molecular weight:14KD
    Observed molecular weight:10~15KD

    Exposure time:7s
    FABP1 Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-Stomach tissue with Rabbit anti-FABP1 antibody (AWA42859) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42859) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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