ISG15 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA42678

应用：

WB,IHC,IF,ELISA

反应性：

Human,Mouse

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse

Molecular Wt:

18 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

G1P2; hUCRP; IFI15; IP17; ISG15; ISG15 ubiquitin like modifier; Ubiquitin like protein ISG15; UCRP; IMD38

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:100-1:300

IF 1:50-1:200

ELISA 1:5000

Immunogen

Information

Gene Name：

ISG15

Protein Name：

Ubiquitin-like protein ISG15

Gene ID：

9636 (Human)

100038882 (Mouse)

SwissPro：

P05161 (Human)

Q64339 (Mouse)

Subcellular Location：

Cytoplasm. Secreted.

Immunogen：

Synthesized peptide derived from part region of human protein.

Specificity:

ISG15 Polyclonal Antibody detects endogenous levels of ISG15 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-hippocampus tissue with Rabbit anti-ISG15 antibody (AWA42678) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42678) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampus tissue with Rabbit anti-ISG15 antibody (AWA42678) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42678) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of ISG15 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42678, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: T24 cell Lane 2: U251 cell Lane 3: GL261 cell Lane 4: Rsc96 cell Lane 5: MCF-7 cell Lane 6: 4T1 cell Lane 7: SHZ-88 cell Lane 8: Hela cell Lane 9: 786-O cell Lane 10: SU-DHL-4 cell Predicted molecular weight:18 kDa Observed molecular weight:18 kDa Exposure time：7 sec