Fibronectin Rabbit Polyclonal Antibody

货号:
AWA42412
应用:
IHC-P,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    272 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    CIG; Cold insoluble globulin; ED B; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    IHC-P 1:50-1:200
    IF 1:50-1:200
    ELISA 1:10000-1:20000



    Immunogen

    Information

    Gene Name:

    FN1

    Protein Name:

    Fibronectin


    Gene ID:

    2335 (Human)
    14268 (Mouse)
    25661 (Rat)

    SwissPro:

    P02751 (Human)    
    P11276 (Mouse)    
    P04937 (Rat)


    Subcellular Location:

    Secreted, extracellular space, extracellular matrix. Secreted.


    Immunogen:

    Synthetic peptide from human Fibronectin. AA range: 2304-2375.


    Specificity:

    Fibronectin Polyclonal Antibody detects endogenous levels of Fibronectin protein.




    Product images
    Fibronectin Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Rat-Kidney tissue with rabbit anti-Fibronectin antibody (AWA42412) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42412) at 1/800 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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