APOC2 Rabbit Polyclonal Antibody

货号:
AWA42393
应用:
WB,IHC-P,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    11 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    APC2; Apo CII; ApoC II; APOC2; apolipoprotein C II; Apolipoprotein C2


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-P 1:100-1:300

    IF 1:50-1:200

    ELISA 1:10000

     



    Immunogen

    Information

    Gene Name:

    APOC2

    Protein Name:

    Apolipoprotein C-II


    Gene ID:

    344 (Human)     

    11813 (Mouse)     

    292697 (Rat)

     

    SwissPro:

    P02655 (Human)     

    Q05020 (Mouse)     

    G3V8D4 (Rat)

     


    Subcellular Location:

    Secreted.


    Immunogen:

    Synthesized peptide derived from human APOC2. AA range: 1-50.


    Specificity:

    APOC2 Polyclonal Antibody detects endogenous levels of APOC2 protein.




    Product images
    APOC2 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of APOC2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42393, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HELA cell
    Lane 2: A431 cell


    Predicted band size: 9 kDa
    Observed band size: 9 kDa

    Exposure time:1min30s
    APOC2 Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-APOC2 antibody (AWA42393) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42393) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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