Prolactin Rabbit Polyclonal Antibody

货号:
AWA42297
应用:
WB,IHC-P,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    26 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    PRL; Prolactin; Decidual prolactin; GHA1; Growth hormone A1; Lactogenic hormone; Luteotropic hormone; Mammotropin; Prolactin precursor

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-P 1:100-1:300

    IF 1:50-1:200

    ELISA 1:20000

     



    Immunogen

    Information

    Gene Name:

    PRL

    Protein Name:

    Prolactin


    Gene ID:

    5617 (Human)     

    19109 (Mouse)

    24683 (Rat)

    SwissPro:

    P01236 (Human)     

    P06879 (Mouse)     

    P01237 (Rat)

     


    Subcellular Location:

    Secreted.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from the Internal region of human Prolactin. AA range: 131-180.

     


    Specificity:

    Prolactin Polyclonal Antibody detects endogenous levels of Prolactin protein.


    Product images
    Prolactin Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Prolactin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42297, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: NIH-3T3 cell
    Lane 2: U87-MG cell
    Lane 3: SHZ-88 cell
    Lane 4: 4T1 cell

    Predicted molecular weight:26kDa
    Observed molecular weight:18kDa

    Exposure time:90s
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