c-Fos Rabbit Polyclonal Antibody

货号:
AWA42269
应用:
WB,IF-C,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    41 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    FOS; FOS protein; G0S7; Proto-oncogene c-Fos; proto oncogene c Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; Activator protein 1; AP 1; Oncogene FOS; p55


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    ELISA 1:10000



    Immunogen

    Information

    Gene Name:

    FOS

    Protein Name:

    Protein c-Fos


    Gene ID:

    2353 (Human)    
    14281 (Mouse)    
    314322 (Rat)

    SwissPro:

    P01100 (Human)    
    P01101 (Mouse)    
    P12841 (Rat)


    Subcellular Location:

    Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human Fos. AA range: 1-50.


    Specificity:

    c-Fos Polyclonal Antibody detects endogenous levels of c-Fos protein.




    Product images
    c-Fos Rabbit Polyclonal Antibody - 1 Fig: Immunocytochemistry analysis of Hela cells labeling C-FOS with Rabbit anti-C-FOS antibody (AWA42269) at 1/50 dilution(green ).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-C-FOS antibody (AWA42269) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    c-Fos Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of c-Fos on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42269, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: Jurkat cell
    Lane 3: K562 cell
    Lane 4: HepG2 cell
    Lane 5: NIH3T3 cell
    Lane 6: RWA 264.7 cell
    Lane 7: HSC-T6 cell

    Predicted molecular weight: 41 kDa
    Observed molecular weight:50-60 kDa

    Exposure time: 180 seconds
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