c-Fos Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 41 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: FOS; FOS protein; G0S7; Proto-oncogene c-Fos; proto oncogene c Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; Activator protein 1; AP 1; Oncogene FOS; p55 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: FOS | Protein Name: Protein c-Fos | ||
Gene ID: 2353 (Human) | SwissPro: P01100 (Human) | |||
Subcellular Location: Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. | ||||
Immunogen: The antiserum was produced against synthesized peptide derived from human Fos. AA range: 1-50. | ||||
Specificity: c-Fos Polyclonal Antibody detects endogenous levels of c-Fos protein. | ||||
Product images | |
Fig: Immunocytochemistry analysis of Hela cells labeling C-FOS with Rabbit anti-C-FOS antibody (AWA42269) at 1/50 dilution(green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-C-FOS antibody (AWA42269) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Western blot analysis of c-Fos on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42269, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Jurkat cell Lane 3: K562 cell Lane 4: HepG2 cell Lane 5: NIH3T3 cell Lane 6: RWA 264.7 cell Lane 7: HSC-T6 cell Predicted molecular weight: 41 kDa Observed molecular weight:50-60 kDa Exposure time: 180 seconds |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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