eIF2α Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
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- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Monkey,Fish | Molecular Wt: 36 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; eIF2α | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: EIF2S1 | Protein Name: Eukaryotic translation initiation factor 2 subunit 1 | ||
Gene ID: 1965 (Human) | SwissPro: P05198 (Human) | |||
Subcellular Location: Cytoplasm, Stress granule. Cytoplasm, cytosol. Mitochondrion. | ||||
Immunogen: The antiserum was produced against synthesized peptide derived from human eIF2α. AA range: 21-70. | ||||
Specificity: eIF2α Polyclonal Antibody detects endogenous levels of eIF2α protein. | ||||
Product images | |
Fig : Western blot analysis of eIF2α on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA42112, 1/3000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SHZ-88 cell lysate Lane 3: 4T1 cell lysate |
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Fig: Immunocytochemistry analysis of Hela cells labeling eIF2α with Rabbit anti-eIF2α antibody (AWA42112)at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-eIF2α antibody (AWA42112)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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