eIF2α Rabbit Polyclonal Antibody

货号:
AWA42112
应用:
WB,IF-C,ELISA
反应性:
Human,Mouse,Rat,Monkey,Fish
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Monkey,Fish

    Molecular Wt:

    36 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    EIF2S1; EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; eIF2α


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    ELISA 1:10000



    Immunogen

    Information

    Gene Name:

    EIF2S1

    Protein Name:

    Eukaryotic translation initiation factor 2 subunit 1


    Gene ID:

    1965 (Human)  
    13665 (Mouse)  
    54318 (Rat)

    SwissPro:

    P05198 (Human)  
    Q6ZWX6 (Mouse)  
    P68101 (Rat)


    Subcellular Location:

    Cytoplasm, Stress granule. Cytoplasm, cytosol. Mitochondrion.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human eIF2α. AA range: 21-70.


    Specificity:

    eIF2α Polyclonal Antibody detects endogenous levels of eIF2α protein.




    Product images
    eIF2α Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of eIF2α on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA42112, 1/3000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: SHZ-88 cell lysate
    Lane 3: 4T1 cell lysate
    eIF2α Rabbit Polyclonal Antibody - 2 Fig: Immunocytochemistry analysis of Hela cells labeling eIF2α with Rabbit anti-eIF2α antibody (AWA42112)at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-eIF2α antibody (AWA42112)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
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