LATS1/2 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA42074

应用：

IHC-P,IF,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

126 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

LATS1; WARTS; Serine/threonine-protein kinase LATS1; LATS2; KPM; h-warts; Large tumor suppressor homolog 1; WARTS protein kinase; Serine/threonine-protein kinase LATS2; Kinase phosphorylated during mitosis protein; Large tumor suppressor ho

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

IHC-P 1:100-1:300

IF 1:100-1:300

ELISA 1:20000

Immunogen

Information

Gene Name：

LATS1/LATS2

Protein Name：

Serine/threonine-protein kinase LATS1/2

Gene ID：

9113/26524 (Human)

16798/50523 (Mouse)

308265 (Rat)

SwissPro：

O95835/Q9NRM7 (Human)

Q8BYR2/Q7TSJ6 (Mouse)

F1M2K4 (Rat)

Subcellular Location：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.

Immunogen：

The antiserum was produced against synthesized peptide derived from human LATS1/2. AA range: 1041-1090.

Specificity:

LATS1/2 Polyclonal Antibody detects endogenous levels of LATS1/2 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Brain tissue with Rabbit anti-LATS1/2 antibody (AWA42074) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-duodenum tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-LATS antibody (AWA42074) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42074) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-LATS1/2 antibody (AWA42074) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42074) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.