LATS1/2(Phospho Ser909) Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA42070

应用：

WB,IHC,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 126 kDa

Observed MW: 100-130 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

LATS1; WARTS

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:50-1:300

ELISA 1:2000-1:20000

Immunogen

Information

Gene Name：

LATS1/LATS2

Protein Name：

Serine/threonine-protein kinase LATS1/2

Gene ID：

9113/26524 (Human)

16798/50523 (Mouse)

308265 (Rat)

SwissPro：

O95835/Q9NRM7 (Human)

Q8BYR2/Q7TSJ6 (Mouse)

F1M2K4 (Rat)

Subcellular Location：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.

Immunogen：

Synthesized phosho peptide around human LATS1/2 (Ser909).

Specificity:

Phospho LATS1/2 (Ser909) Polyclonal Antibody detects endogenous levels of Phospho LATS1/2 (Ser909) protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Kidney tissue with Rabbit anti-LATS1/2(Phospho Ser909) antibody (AWA42070) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42070) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of LATS1/2(Phospho Ser909) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42070, 1/1500) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: U251 cell Lane 3: MCF-7 cell Lane 4: HepG2 cell Predicted molecular weight:126 kDa Observed molecular weight:100-130 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Brain tissue with Rabbit anti-LATS1/2(Phospho Ser909) antibody (AWA42070) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42070) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Uterus tissue with Rabbit anti-LATS1/2(Phospho Ser909) antibody (AWA42070) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42070) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.