ACSL3 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA41997

应用：

WB,IHC-P,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

80 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ACS3; ACSL3; FACL3; LACS 3; LACS3; PRO2194

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-1:300

ELISA 1:5000-1:20000

Immunogen

Information

Gene Name：

ACSL3

Protein Name：

Fatty acid CoA ligase Acsl3

Gene ID：

2181 (Human)

74205 (Mouse)

114024 (Rat)

SwissPro：

O95573 (Human)

Q9CZW4 (Mouse)

Q63151 (Rat)

Subcellular Location：

Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.

Immunogen：

Synthesized peptide derived from human protein. AA range: 40-120.

Specificity:

ACSL3 Polyclonal Antibody detects endogenous levels of ACSL3 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-large intestine tissue with Rabbit anti-ACSL3 antibody (AWA41997) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41997) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of ACSL3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA41997, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293T cell Lane 2: HEPG2 cell Lane 3: K562 cell Lane 4: Hela cell
	Fig : Western blot analysis of ACSL3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA41997, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NRK-49F cell Lane 2: RM-1 cell Lane 3: AML12 cell Lane 4: PC3 cell Predicted molecular weight: 80KD Observed molecular weight: 80KD
	Fig: Fluorescence immunohistochemical analysis of mouse-vas deferens tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-ACSL3 antibody (AWA41997) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41997) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.