ABCA1 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA41977

应用：

WB,IHC,ELISA

反应性：

Human,Mouse,Rat,Golden hamster

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,

Golden hamster

Molecular Wt:

254 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ABC 1; ABC1; ABCA1; ATP binding cassette 1; CERP; HDLDT1; TGD

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:50-1:300

ELISA 1:5000-1:20000

Immunogen

Information

Gene Name：

ABCA1

Protein Name：

Phospholipid-transporting ATPase ABCA1

Gene ID：

19 (Human)

11303 (Mouse)

313210 (Rat)

SwissPro：

O95477 (Human)

P41233 (Mouse)

Q80ZB2 (Rat)

Subcellular Location：

Cell membrane. Endosome.

Immunogen：

Synthesized peptide derived from part region of human protein. AA range: 1112-1180.

Specificity:

ABCA1 Polyclonal Antibody detects endogenous levels of ABCA1 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-ABCA1 (AWA41977) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41977) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intestine tissue with Rabbit anti-ABCA1 (AWA41977) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41977) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of ABCA1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA41977, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: AML12 cell Lane 2: U87 cell Lane 3: LN229 cell Lane 4: N-2A cell Lane 5: HT-22 cell Lane 6: Rat brain tissue Lane 7: RSC96 cell Lane 8: NRK-49F cell Lane 9: PC12 cell Predicted molecular weight:254 kDa Observed molecular weight:254 kDa