CEBPB Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA41837

应用：

WB,IF,ELISA

反应性：

Human,Mouse,Rat,Pig

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Pig

Molecular Wt:

36 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CEBPB; LAP; TCF5; PP9092; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; Nuclear factor NF-IL6; Transcription factor 5; TCF-5

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IF 1:50-1:200

ELISA 1:10000

Immunogen

Information

Gene Name：

CEBPB

Protein Name：

CCAAT/enhancer-binding protein beta

Gene ID：

1051 (Human)

12608 (Mouse)

24253 (Rat)

SwissPro：

P17676 (Human)

P28033 (Mouse)

P21272 (Rat)

Subcellular Location：

Nucleus. Cytoplasm.

Immunogen：

The antiserum was produced against synthesized peptide derived from human CEBPB. AA range: 200-256.

Specificity:

CEBPB Polyclonal Antibody detects endogenous levels of CEBPB protein.

Product images
	Fig: Immunocytochemistry analysis of NIH/3T3 cells labeling CEBPB with Rabbit anti-CEBPB antibody （AWA41837）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-CEBPB antibody （AWA41837）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of CEBPB on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA41837, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: RAW264.7 cell