ATG12 Rabbit Polyclonal Antibody

货号:
AWA41775
应用:
WB
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    15 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    APG12; APG12 like; APG12L; ATG12; Autophagy related protein 12; FBR93; HAPG12; Ubiquitin like protein ATG12

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography

     


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000



    Immunogen

    Information

    Gene Name:

    ATG12

    Protein Name:

    Ubiquitin-like protein ATG12


    Gene ID:

    9140 (Human)

    67526 (Mouse)

    361321 (Rat)

     

    SwissPro:

    O94817 (Human)     

    Q9CQY1 (Mouse)     

    Q2TBJ5 (Rat)

     


    Subcellular Location:

    Cytoplasm. Preautophagosomal structure membrane.

     


    Immunogen:

    Synthesized peptide derived from human ATG12. AA range: 80-130.


    Specificity:

    ATG12 Polyclonal Antibody detects endogenous levels of ATG12 protein.


    Product images
    ATG12 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of ATG12 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA41775, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SH-SY5Y cell
    Lane 2: A431 cell
    Lane 3: HEK293 cell
    Lane 4: K562 cell
    Lane 5: NIH3T3 cell
    Lane 6: PC12 cell
    Lane 7: Rat brain tissue


    Predicted molecular weight: 15 kDa
    Observed molecular weight: 17-20 kDa (Free ATG12),48-55 kDa (Conjugated to ATG5, ATG12-ATG5 )

    Exposure time: 180 seconds
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