ULK1(Phospho Ser555)Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA41517

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

113 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ATG1; FLJ38455; FLJ46475; KIAA0722; Phospho-ULK1 (Ser555); ULK1; Unc 51 like kinase 1; UNC51; Unc51.1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:2000

IHC-P 1:100-1:300

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

ULK1

Protein Name：

Serine/threonine-protein kinase ULK1

Gene ID：

8408 (Human)

22241 (Mouse)

360827 (Rat)

SwissPro：

O75385 (Human)

O70405 (Mouse)

A6J290 (Rat)

Subcellular Location：

Cytoplasm, cytosol. Preautophagosomal structure.

Immunogen：

Synthesized phosho peptide around human ULK1 (Ser555).

Specificity:

Phospho ULK1 (Ser555) Polyclonal Antibody detects endogenous levels of Phospho ULK1 (Ser555) protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-uterus intestine tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-ULK1(Phospho Ser555) antibody (AWA41517) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 7.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-ULK1(Phospho Ser555) antibody (AWA41517) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-small intestine tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-ULK1(Phospho Ser555) antibody (AWA41517) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 7.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-myocardium tissue with Rabbit anti-ULK1(Phospho Ser555) antibody (AWA41517) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Rat-spleen intestine tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-ULK1(Phospho Ser555) antibody (AWA41517) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 7.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA41517) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.