GSDME N-terminal Rabbit Polyclonal Antibody

货号:
AWA41251
应用:
WB,IHC,IF
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    36,55 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    Gasdermin-E; DFNA5; Dfna5h; EG14210,2310037D07Rik; 4932441K13Rik; ICERE 1; ICERE-1; Deafness; autosomal dominant 5; Non-syndromic hearing impairment protein 5; GSDME-NT

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-20000

    IHC 1:200-1:300

    IF 1:50-1:200

     



    Immunogen

    Information

    Gene Name:

    GSDME

    Protein Name:

    Gasdermin-E


    Gene ID:

    1687 (Human)     

    54722 (Mouse)     

    353316 (Rat)

     

    SwissPro:

    O60443 (Human)     

    Q9Z2D3 (Mouse)     

    A0A8I5ZRE3 (Rat)

     


    Subcellular Location:

    Cell membrane. Cytoplasm, cytosol.


    Immunogen:

    Synthesized peptide derived from human GSDME. AA range: 1-100.


    Specificity:

    GSDME N-terminal Polyclonal Antibody detects endogenous levels of GSDME N-terminal protein.




    Product images
    GSDME N-terminal Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of GSDME N-terminal on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA41251, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse kidney tissue
    Lane 2: Hela cell treated with 20ng/ml hTNF-αfor 5min
    Lane 3: CAL27 cell

    Predicted molecular weight:35 kDa(GSDME N-terminal),55KD(Full length)
    Observed molecular weight:35 kDa(GSDME N-terminal),55KD(Full length)

    Exposure time:3 min
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