CD133 Rabbit Polyclonal Antibody

货号:
AWA40999
应用:
WB,IF,ELISA
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    97 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    PROM1; Prominin-1; Antigen AC133; Prominin-like protein 1; CD133


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IF 1:100-1:300

    ELISA 1:20000

     



    Immunogen

    Information

    Gene Name:

    PROM1

    Protein Name:

    Prominin-1


    Gene ID:

    8842 (Human)     

    19126 (Mouse)     

     

    SwissPro

    O43490 (Human)     

    O54990 (Mouse)     

     


    Subcellular Location:

    Endoplasmic reticulum. Plasma membrane. Cell projection.


    Immunogen

    The antiserum was produced against synthesized peptide derived from the N-terminal region of human PROM1. AA range: 41-90.

     


    Specificity:

    CD133 Polyclonal Antibody detects endogenous levels of CD133 protein.




    Product images
    CD133 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of CD133 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA40999, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AAWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HT-29 cell
    Lane 2: HepG2 cell
    Lane 3: CT26 cell
    Lane 4: AML12 cell
    Lane 5: HepA1-6 cell
    Lane 6: RSC96 cell
    Lane 7: LOVO


    Predicted molecular weight:97 kDa
    Observed molecular weight:97 kDa
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