PKR(Phospho Thr446) Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 62 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: eIF 2A protein kinase 2; EIF2AK2; EIF2AK2; PKR; P1/eIF 2A protein kinase; p68 kinase; PKR; PRKR; Protein kinase RNA activated; DYT33; LEUDEN; EIF2AK1; PPP1R83; PKR/EIF2AK2; PKR(Phospho Thr446) | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： EIF2AK2 | Protein Name： Interferon-induced, double-stranded RNA-activated protein kinase | ||
Gene ID： 5610 (Human) | SwissPro： 5610 (Human) | |||
Subcellular Location： Cytoplasm. Nucleus. Cytoplasm, perinuclear region. | ||||
Immunogen： The antiserum was produced against synthesized peptide derived from human PKR around the phosphorylation site of Thr446. AA range: 413-462. | ||||
Specificity: Phospho PKR (Thr446) Polyclonal Antibody detects endogenous levels of PKR protein. only when phosphorylated at Thr446. | ||||
| Product images | |
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Fig: Fluorescence immunohistochemical analysis of Mouse-bladder tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-PKR(Phospho Thr446) antibody (AWA40532) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40532) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded mouse-colon tissue with rabbit anti-PKR(Phospho Thr446) antibody (AWA40532) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40532) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Western blot analysis of PKR(Phospho Thr446) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA40532, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1:THP-1 cell Lane 2: SH-SY5Y cell Lane 3: MCF-7 cell Lane 4: C2C12 cell Lane 5: K562 cell Lane 6: HEPG2 cell Lane 7: A549 cell Lane 8: Jurkat cell Lane 9: HELA cell |
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Fig : Immunohistochemical analysis of paraffin-embedded rat-brain tissue with rabbit anti-PKR(Phospho Thr446) antibody (AWA40532) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40532) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Rat-Liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-PKR(Phospho Thr446) antibody (AWA40532) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40532) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded rat-kidney tissue with rabbit anti-PKR(Phospho Thr446) antibody (AWA40532) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40532) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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