Collagen IV Rabbit Polyclonal Antibody

货号:
AWA40461
应用:
IHC-P,IF
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    161 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    Collagen IV; CollagenIV; Arresten; Collagen alpha-1(IV) chain; BSVD; BSVD1; RATOR; PADMAL; COL4A1s; COL4A1


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    IHC-P 1:100-1:1000
    IF 1:50-1:200



    Immunogen

    Information

    Gene Name:

    COL4A1

    Protein Name:

    Collagen alpha-1(IV) chain


    Gene ID:

    1282 (Human)
    12826 (Mouse)
    290905 (Rat)

    SwissPro:

    P02462 (Human)
    P02463 (Mouse)
    F1MA59 (Rat)


    Subcellular Location:

    Secreted, extracellular space, extracellular matrix, basement membrane.


    Immunogen:

    Recombinant protein within C-terminal human Collagen IV.


    Specificity:

    Collagen IV Polyclonal Antibody detects endogenous levels of Collagen IV protein.




    Product images
    Collagen IV Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-muscle tissue with Rabbit anti-Collagen IV (AWA40461) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40461) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Collagen IV Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-Kidney tissue with rabbit anti-Collagen IV antibody (AWA40461) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA40461) at 1/800 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg