SFRS6 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA13603

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

40 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

B52; Pre mRNA splicing factor SRP55; SFRS6; SRP55; SRSF6; HEL-S-91

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:50-1:200

Immunogen

Information

Gene Name：

SRSF6

Protein Name：

Serine/arginine-rich splicing factor 6

Gene ID：

6431 (Human)

67996 (Mouse)

362264 (Rat)

SwissPro：

Q13247 (Human)

Q3TWW8 (Mouse)

G3V6S8 (Rat)

Subcellular Location：

Nucleus. Nucleus speckle.

Immunogen：

Synthetic peptide within human SFRS6. AA range: 150-199.

Specificity:

SFRS6 Monoclonal Antibody detects endogenous levels of SFRS6 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-jejunum tissue with Rabbit anti-SFRS6 antibody (AWA13603) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA13603) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of SFRS6 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13603, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell Lane 2: Hela cell Lane 3: A431 cell Lane 4: Raw264.7 cell Lane 5: SH-SY5Y cell Lane 6: Jurkat cell Lane 7: MCF-7 cell Lane 8: SK-BR-3 cell Lane 9: THP-1 cell Predicted molecular weight:40 kDa Observed molecular weight:53 kDa; Exposure time: 45seconds