NSUN2 Recombinant Rabbit Monoclonal Antibody

货号:
AWA13426
应用:
WB,IHC-P,IF-C,IF-T,IP
反应性:
Human,Mouse,Monkey
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Monkey

    Molecular Wt:

    86 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    SAKI; RNA cytosine C(5)-methyltransferase NSUN2; Myc-induced SUN domain-containing protein; mRNA cytosine C(5)-methyltransferase; EC:2.1.1.203; 5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; MISU; MRT5; TRM4; NSUN2

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:2000

    IHC-P 1:200-1:1000

    IF-C 1:50-1:250

    IF-T 1:200

    IP 1-2μg/sample 

     



    Immunogen

    Information

    Gene Name:

    NSUN2

    Protein Name:

    RNA cytosine C(5)-methyltransferase NSUN2


    Gene ID:

    54888 (Human)     

    28114  (Mouse)     

     

    SwissPro:

    Q08J23 (Human)     

    Q1HFZ0 (Mouse)     

     


    Subcellular Location:

    Nucleus, nucleolus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, spindle. Secreted, extracellular exosome. 

     


    Immunogen:

    Recombinant protein within human NSUN2. AA range: 1-767.


    Specificity:

    NSUN2 Monoclonal Antibody detects endogenous levels of NSUN2 protein.


    Product images
    NSUN2 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of NSUN2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13426, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell
    Lane 2: Hela cell
    Lane 3: MCF-7 cell

    Predicted molecular weight: 86 kDa
    Observed molecular weight: ~86 kDa
    Exposure time: 15s
    NSUN2 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of NSUN2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13426, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell
    Lane 2: HT1080 cell
    Lane 3: Caco2 cell
    Lane 4: N-2a cell

    Predicted molecular weight: 86 kDa
    Observed molecular weight: ~86 kDa
    Exposure time: 3min
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