YB-1/YBX1 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA13326

应用：

WB,IHC-P,IF-C,IF-T,FCM,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

36 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

YB 1; YB1; YBX 1; BP 8; BP-8; CSDB; DBPB; YB-1; CBF-A; CSDA2; EFI-A; NSEP 1; NSEP1; NSEP-1; MDR NF1; MDR-NF1; YB-1/YBX1; CCAAT binding transcription factor I subunit A; DNA binding protein B; Enhancer factor I subunit A

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:50-1:200

IF-C 1:100-1:500

IF-T 1:100-1:500

FCM 1:50-1:100

IP Use at an assay dependent concentration.

Immunogen

Information

Gene Name：

YBX1

Protein Name：

Y-box-binding protein 1

Gene ID：

4904 (Human)

22608 (Mouse)

500538 (Rat)

SwissPro：

P67809 (Human)

P62960 (Mouse)

P62961 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome. Cytoplasm, P-body.

Immunogen：

Synthetic peptide within human YB-1/YBX1. AA range: 275-324.

Product images
	Fig : Western blot analysis of YB-1/YBX1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13326, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart Lane 2: LLC cell Predicted molecular weight: 36 kDa Observed molecular weight: 36~55 kDa Exposure time：3min
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-YB-1 antibody (AWA13326) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA13326) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.