PSMB9 Recombinant Rabbit Monoclonal Antibody

货号:
AWA13049
应用:
WB,IHC-P,IP
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    23 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    LMP2; LMP 2; PSMB9; PSMB 9; PRAAS3; PSMB6i; RING12; RING 12; RING12 protein; beta1i; PSMB9/LMP2; Large multifunctional peptidase 2; Low molecular mass protein 2; Macropain chain 7; Proteasome beta 9 subunit

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:1000

    IHC-P 1:50-1:200

    IP Use at an assay dependent concentration.

     



    Immunogen

    Information

    Gene Name:

    PSMB9

    Protein Name:

    Proteasome subunit beta type-9


    Gene ID:

    5698 (Human)     

    16912 (Mouse)     

     

    SwissPro:

    P28065 (Human)     

    P28076 (Mouse)     

     


    Subcellular Location:

    Cytoplasm. Nucleus. 


    Immunogen:

    Recombinant protein within human PSMB9. AA range: 85-219.


    Specificity:

    PSMB9 Monoclonal Antibody detects endogenous levels of PSMB9 protein.


    Product images
    PSMB9 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of PSMB9 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse kidney
    Lane 2: Mouse lung
    Lane 3: J774A.1 cell
    Lane 4: Raw264.7 cell

    Predicted molecular weight: 23 kDa
    Observed molecular weight: 23 kDa
    Exposure time: 15s
    PSMB9 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of PSMB9 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell
    Lane 2: THP-1 cell
    Lane 3: Jurkat cell
    Lane 4: Raji cell
    Lane 5: Rat liver
    Lane 6: Daudi cell
    Lane 7: RBL-2H3 cell
    Lane 8: U937 cell
    Lane 9: Rat lung
    Lane 10: Mouse liver

    Predicted molecular weight: 23 kDa
    Observed molecular weight: 23 kDa
    Exposure time: 15s
    PSMB9 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Western blot analysis of PSMB9 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13049, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HL60 cell
    Lane 2: 3D4/21 cell
    Lane 3: COS7 cell

    Predicted molecular weight: 23 kDa
    Observed molecular weight: 23 kDa
    Exposure time: 45s
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