ATP5A1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 60 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: ATP5F1A | Protein Name: ATP synthase subunit alpha, mitochondrial | ||
Gene ID: 498 (Human) | SwissPro: P25705 (Human) | |||
Subcellular Location: Mitochondrion. Mitochondrion inner membrane. Cell membrane. | ||||
Immunogen: Synthetic peptide within human ATP5A1. AA range: 191-234. | ||||
Specificity: ATP5A1 Monoclonal Antibody detects endogenous levels of ATP5A1 protein. | ||||
Product images | |
Fig : Western blot analysis of ATP5A1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13035, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain Lane 2: Rat brain Lane 3: HepG2 cell Lane 4: Jurkat cell Lane 5: A549 cell Lane 6: Mcf-7 cell Lane 7: Hek293 cell Lane 8: HL-60 cell Predicted molecular weight:60 kDa Observed molecular weight:50 kDa Exposure time:45s | |
Fig : Western blot analysis of ATP5A1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA13035, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat heart Lane 2: Mouse heart Predicted molecular weight:60 kDa Observed molecular weight:50 kDa Exposure time:7s | |
Fig: Fluorescence immunohistochemical analysis of Rat-cerebrum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-ATP5A1 antibody (AWA13035) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA13035) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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