ATF6 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12712
应用:
WB,IHC-P,ChIP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    75 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    ATF6; ATF 6; ATF6 alpha; ATF6-alpha; ATF6AZ; Activating transcription factor 6 alpha; Activating transcription factor 6; ATF6A; ATF6A_HUMAN; ESTM49; FLJ21663

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000

    IHC-P 1:200-1:1000

    ChIP Use 0.5~2 μg for 25 μg of chromatin. 

     



    Immunogen

    Information

    Gene Name:

    ATF6

    Protein Name:

    Cyclic AMP-dependent transcription factor ATF-6 alpha

     


    Gene ID:

    22926 (Human)     

    226641 (Mouse)     

    304962 (Rat)

     

    SwissPro:

    P18850 (Human)     

    F6VAN0 (Mouse)     

    G3V909 (Rat)

     


    Subcellular Location:

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus.


    Immunogen:

    Recombinant protein within human ATF6. AA range: 1-350.


    Specificity:

    ATF6 Monoclonal Antibody detects endogenous levels of ATF6 protein.


    Product images
    ATF6 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of ATF6 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12712, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Ribbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2:A431 cell
    Lane 3: HEK293 cell
    Lane 4:Mouse brain
    Lane 5:HUVEC cell
    Lane 6:PC12 cell
    Lane 7:RBL-2H3

    Predicted molecular weight:75 kDa
    Observed molecular weight:110 kDa;

    Exposure time: 45seconds
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