IFN-γ Rabbit Polyclonal Antibody
-
-
- 20μL
- ¥620
- 有库存
-
- 50μL
- ¥1250
- 有库存
-
- 100μL
- ¥2200
- 有库存
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 17 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: IFNG; Interferon gamma; IFN-gamma; Immune interferon | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:500-1:2000 IHC 1:100-1:300 IF 1:50-1:200 ELISA 1:40000
| |||
Immunogen Information | Gene Name: IFNG | Protein Name: Interferon gamma | ||
Gene ID: 3458 (Human) 15978 (Mouse) 25712 (Rat) | SwissPro: P01579 (Human) P01580 (Mouse) P01581 (Rat)
| |||
Subcellular Location: Secreted. | ||||
Immunogen: The antiserum was produced against synthesized peptide derived from human IFN-gamma. AA range:20-69.
| ||||
Specificity: IFN-γ Polyclonal Antibody detects endogenous levels of IFN-γ protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-testistissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Western blot analysis of IFN-γ on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA42324, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: HEPA1-6 cell lysate Lane 3: AML12 cell lysate Lane 4: RAW264.7(RAW 264.7 treated with 100 ng/ml LPS for 7 h and 300 ng/ml Brefeldin A for the last 3h) cell lysate Predicted molecular weight: 19.3KD Observed molecular weight: 24KD |
|
Fig : Immunohistochemical analysis of paraffin-embedded mouse-lung tissue with rabbit anti-IFN-γ antibody (AWA42324) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/800 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
相关产品
-
Cdk6 Recombinant Rabbit Monoclonal Antibody
GAPDH Rabbit Polyclonal Antibody
GFAP Recombinant Mouse Monoclonal Antibody
Ki67 Rabbit Monoclonal Antibody
HMGB1 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Mouse Monoclonal Antibody
p53 Recombinant Rabbit Monoclonal Antibody
Bcl-2 Recombinant Rabbit Monoclonal Antibody