IFN-γ Rabbit Polyclonal Antibody

货号:
AWA42324
应用:
WB,IHC,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 有库存
  • 50μL 
  • ¥1250
  • 有库存
  • 100μL 
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    17 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    IFNG; Interferon gamma; IFN-gamma; Immune interferon


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC 1:100-1:300

    IF 1:50-1:200

    ELISA 1:40000  

     



    Immunogen

    Information

    Gene Name:

    IFNG

    Protein Name:

    Interferon gamma


    Gene ID:

    3458 (Human)     

    15978 (Mouse)     

    25712 (Rat)

    SwissPro

    P01579 (Human)     

    P01580 (Mouse)     

    P01581 (Rat)

     


    Subcellular Location:

    Secreted.


    Immunogen

    The antiserum was produced against synthesized peptide derived from human IFN-gamma. AA range:20-69.

     


    Specificity:

    IFN-γ Polyclonal Antibody detects endogenous levels of IFN-γ protein.




    Product images
    IFN-γ Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    IFN-γ Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    IFN-γ Rabbit Polyclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-testistissue with Rabbit anti-IFN-γ (AWA42324) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    IFN-γ Rabbit Polyclonal Antibody - 4 Fig : Western blot analysis of IFN-γ on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA42324, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell lysate
    Lane 2: HEPA1-6 cell lysate
    Lane 3: AML12 cell lysate
    Lane 4: RAW264.7(RAW 264.7 treated with 100 ng/ml LPS for 7 h and 300 ng/ml Brefeldin A for the last 3h) cell lysate

    Predicted molecular weight: 19.3KD
    Observed molecular weight: 24KD
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