ERK1/2 Recombinant Rabbit Monoclonal Antibody
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-
- 20μL
- ¥620
- 有库存
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- 50μL
- ¥1250
- 有库存
-
- 100μL
- ¥2200
- 有库存
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Zebrafish | Molecular Wt: 43,41 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2; MAPK3; MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:5000 IHC-P 1:20000 IHC-F 1:50 IF-C 1:2000 IF-T 1:50 FCM 1:1000 IP Use at an assay dependent concentration.
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Immunogen Information | Gene Name: MAPK1/MAPK3 | Protein Name: Mitogen-activated protein kinase 1/Mitogen-activated protein kinase 3
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Gene ID: 5595/5594 (Human) 26417/26413 (Mouse) 50689/116590 (Rat) | SwissPro: P27361/P28482 (Human) P63085/Q63844 (Mouse) P21708/P63086 (Rat)
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Subcellular Location: Cytoplasm, Nucleus. | ||||
Immunogen: Recombinant protein within human ERK2. AA range:180-379. | ||||
Specificity: ERK1/2 Monoclonal Antibody detects endogenous levels of ERK1/2 protein. | ||||
Product images | |
Fig : Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11935, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U251 cell Lane 2: N-2A cell Lane 3: RBL-2H3 cell Lane 4:PC-12 cell Lane 5: HELA cell Lane 6: NPK-49F cell Lane 7: HEK-293 cell Lane 8: LLC cell Predicted band size: 43kDa Observed band size: 38-44kDa |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-muscle tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intestine tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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