ERK1/2 Recombinant Rabbit Monoclonal Antibody

货号：

AWA11935

应用：

WB,IHC-P,IHC-F,IF-C,IF-T,IP,FCM

反应性：

Human,Mouse,Rat,Zebrafish,Rabbit

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Zebrafish,Rabbit

Molecular Wt:

Predicted MW: 41/43 kDa
Observed MW: 41/43 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

ERK1; ERK; ERT2; ERK-1; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; 5594/5595; ERK1/2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1;100-1:1000
IHC-F 1:100-1:500
IF-C 1:50-1:200
IF-T 1:100-1:500
IP 1-2ug/sample
FCM 1:50-1:200

Immunogen
Information

Gene Name:

MAPK1/MAPK3

Protein Name:

Mitogen-activated protein kinase 1/Mitogen-activated protein kinase 3

Gene ID:

5594/5595 (Human)
26413/26417 (Mouse)
116590/50689 (Rat)

SwissPro:

P28482/P27361 (Human)
P63085/Q63844 (Mouse)
P63086/P21708 (Rat)

Subcellular Location:

Cytoplasm, cytoskeleton, spindle. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Membrane, caveola. Cell junction, focal adhesion.

Immunogen:

Recombinant protein within human ERK1/2. AA range: 180-379.

Specificity:

ERK1/2 Monoclonal Antibody detects endogenous levels of ERK1/2 protein.

Product images
	Fig：Flow cytometric analysis of C2C12 cells labeling ERK1/2. Overlay histogram showing C2C12 cells stained with ERK1/2 (green line). The cells were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA75001, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody (AWS0005) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
	Fig : Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11935, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U251 cell Lane 2: N-2A cell Lane 3: RBL-2H3 cell Lane 4:PC-12 cell Lane 5: HELA cell Lane 6: NPK-49F cell Lane 7: HEK-293 cell Lane 8: LLC cell Predicted band size: 41/43 kDa Observed band size: 41/43 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-muscle tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intestine tissue with Rabbit anti-ERK1-2 (AWA11935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.