NFκB-p65 Mouse Monoclonal Antibody

货号:
AWA00848
应用:
WB,IF-C,ELISA
反应性:
Human,Mouse
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse

    Molecular Wt:

    60 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    1mg/ml


    Other Names:

    RELA; NFKB3; Transcription factor p65; Transcription factor NFKB3; Nuclear factor NF-kappa-B p65 subunit; relA; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFkappaB p65; p65; NFkB p65; Nuclear factor kappaB; p65 NF kappaB; p65 NFkB


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    ELISA 1:1000-1:5000



    Immunogen

    Information

    Gene Name:

    RELA

    Protein Name:

    Transcription factor p65


    Gene ID:

    5970 (Human)
    19697 (Mouse)
    309165 (Rat)

    SwissPro:

    Q04206 (Human)  
    Q04207 (Mouse)  
    Q7TQN4 (Rat)


    Subcellular Location:

    Nucleus. Cytoplasm.


    Immunogen:

    Recombinant Protein of human NFκB-p65. AA range: 200-300.


    Specificity:

    NFκB-p65 Monoclonal Antibody detects endogenous levels of NFκB-p65 protein.




    Product images
    NFkB p65 Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of NFκB-p65 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00848, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HELA cell
    Lane 2: HEK-293 cell
    Lane 3: Jurkat cell
    Lane 4: RAW264.7 cell
    Lane 5: NIH3T3 cell
    NFkB p65 Mouse Monoclonal Antibody - 2 Fig: Immunocytochemistry analysis of NIH/3T3 cells labeling NFκB-p65 with Mouse anti-NFκB-p65 antibody (AWA00848) at 1/50 dilution(Red).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-NFkB P65 antibody (AWA00848) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 567, AWS0004c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
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