NFκB-p65 Mouse Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
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- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Mouse | Reactivity: Human,Mouse | Molecular Wt: 60 kDa | |
Clonality: Monoclonal | Isotype: IgG2a | Concentration: 1mg/ml | ||
Other Names: RELA; NFKB3; Transcription factor p65; Transcription factor NFKB3; Nuclear factor NF-kappa-B p65 subunit; relA; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFkappaB p65; p65; NFkB p65; Nuclear factor kappaB; p65 NF kappaB; p65 NFkB | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: RELA | Protein Name: Transcription factor p65 | ||
Gene ID: 5970 (Human) | SwissPro: Q04206 (Human) | |||
Subcellular Location: Nucleus. Cytoplasm. | ||||
Immunogen: Recombinant Protein of human NFκB-p65. AA range: 200-300. | ||||
Specificity: NFκB-p65 Monoclonal Antibody detects endogenous levels of NFκB-p65 protein. | ||||
Product images | |
Fig : Western blot analysis of NFκB-p65 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00848, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HELA cell Lane 2: HEK-293 cell Lane 3: Jurkat cell Lane 4: RAW264.7 cell Lane 5: NIH3T3 cell |
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Fig: Immunocytochemistry analysis of NIH/3T3 cells labeling NFκB-p65 with Mouse anti-NFκB-p65 antibody (AWA00848) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-NFkB P65 antibody (AWA00848) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 567, AWS0004c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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