SQSTM1/p62 Mouse Monoclonal Antibody

货号：

AWA00005

应用：

IF-C,FCM

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details	Host Species: Mouse	Reactivity: Human,Mouse,Rat		Molecular Wt: 48 kDa
	Clonality: Monoclonal	Isotype: IgG1		Concentration: 1mg/ml
	Other Names: P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62
	Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
	Purification: Affinity-chromatography
	Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications	IF-C 1:50-1:400 FCM 1:50

Immunogen Information	Gene Name： SQSTM1		Protein Name： Sequestosome-1
	Gene ID： 8878 (Human) 18412 (Mouse) 113894 (Rat)		SwissPro： Q13501 (Human) Q64337 (Mouse) O08623 (Rat)
	Subcellular Location： Cytoplasmic vesicle, autophagosome. Preautophagosomal structure. Cytoplasm, cytosol. Nucleus, PML body. Late endosome. Lysosome. Nucleus. Endoplasmic reticulum. Cytoplasm, myofibril, sarcomere.
	Immunogen： Synthetic peptide within human SQSTM1/p62. AA range: 42-91.
	Specificity: SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.

Product images

SQSTM1/p62 Mouse Monoclonal Antibody - 1

Fig: Immunocytochemistry analysis of Hela cells labeling SQSTM1/p62 with mouse anti-SQSTM1/p62 antibody (AWA00005) at 1/200 dilution(Red).

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with mouse anti-SQSTM1（p62） antibody （AWA00005）at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-mouse IgG H&L (iFluor™ 594, AWS0004c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).