β-actin Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80113

应用：

WB,IHC-P,IF,FCM,IP

反应性：

Human,Mouse,Rat,Pig,Monkey, Rabbit,Goat,Zebrafish,Dog, Monkey,Chicken,Cow,Hamster

来源：

Rabbit

规格 价格 库存
50μL ￥580 1-3个工作日
100μL ￥920 1-3个工作日
500μL ￥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Pig,Monkey,

Rabbit,Goat,Zebrafish,Dog,

Monkey,Chicken,Cow,Hamster

Molecular Wt:

42 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β; Actin Beta; β-Actin; Beta-Actin; Beta actin; PS1TP5BP1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:10000-1:50000

IHC-P 1:200-1:1000

IF 1:100-1:250

FCM 1:1000

IP 1-2μg/sample

Immunogen

Information

Gene Name：

ACTB

Protein Name：

Actin, cytoplasmic 1

Gene ID：

60 (Human)

11461 (Mouse)

81822 (Rat)

SwissPro：

P60709 (Human)

P60710 (Mouse)

P60711 (Rat)

Subcellular Location：

Cytoplasm, cytoskeleton. Nucleus.

Immunogen：

Synthetic peptide within N-terminal residues of β-actin.

Specificity:

β-actin Monoclonal Antibody detects endogenous levels of β-actin protein.

Product images
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80113, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: U251 cell lysate Lane 5: HepG2 cell lysate Lane 6: HUVEC cell lysate Lane 7: LLC cell lysate Lane 8: LLC cell lysate Lane 9: NRK-49F cell lysate Lane 10: EL-4-B5 cell lysate Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 3 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-thymus tissue with Rabbit anti-β-actin antibody (AWA80113) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80113) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-uterus tissue with Rabbit anti-β-actin antibody (AWA80113) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80113) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80113, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 21: RBL-2H3 cell lysate Lane 22: SHZ-88 cell lysate Lane 23: PC-12 cell lysate Lane 24: 4T1 cell lysate Lane 25: HT22 cell lysate Lane 26: N-2A cell lysate Lane 27: COS7 cell lysate Lane 28: 3D4/21 cell lysate Lane 29: Mouse spleen tissue lysate Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 3 seconds
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80113, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 11: MC38 cell lysate Lane 12: GL261 cell lysate Lane 13: PC-9 cell lysate Lane 14: A431 cell lysate Lane 15: HL-60 cell lysate Lane 16: HT-29 cell lysate Lane 17: SK-N-SH cell lysate Lane 18: K562 cell lysate Lane 19: SH-SY5Y cell lysate Lane 20: Raji cell lysate Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 3 seconds