α-Tubulin Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80112

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat,Zebrafish

来源：

Rabbit

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Zebrafish

Molecular Wt:

50 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

TUBA1; TUBA1A; TUBA2; TUBA3; TUBA3C; TUBA4A; Tubulin, alpha 1a; Tubulin, alpha 3c; Tubulin, alpha 4a; Alpha-tubulin; Alphatubulin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:10000

IHC-P 1:200-1:500

IF-C 1:200-1:500

IF-T 1:200-1:500

FCM 1:50-1:100

Immunogen

Information

Gene Name：

TUBA4A

Protein Name：

Tubulin alpha-4A chain

Gene ID：

7277 (Human)

22145 (Mouse)

316531 (Rat)

SwissPro：

P68366 (Human)

P68368 (Mouse)

Q5XIF6 (Rat)

Subcellular Location：

Cytoplasm, Cytoskeleton, Microtubule.

Immunogen：

Synthetic peptide within Human α-Tubulin. AA range: 402-451.

Specificity:

α-Tubulin Polyclonal Antibody detects endogenous levels of α-Tubulin protein.

Product images
	Fig : Western blot analysis of α-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80112, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raw264.7 cell Lane 2: Hela cell Lane 3: NIH3T3 cell Lane 4: PC12 cell Lane 5: Rat brain Lane 6: Hek293 cell Lane 7: A431 cell Lane 8: K562 cell Lane 9: Jurkat cell Exposure time: 7 seconds Predicted band size: 50 kDa Observed band size: 52 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Rabbit anti-α-Tubulin (AWA80112) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-α-Tubulin (AWA80112) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.