α-Tubulin Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80111

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
50μL ¥580 1-3个工作日
100μL ¥920 1-3个工作日
500μL ¥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

55 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Alpha tubulin; Alpha-tubulin; Alpha-tubulin 1; ALS22; B ALPHA 1; bA408E5.3; H2 ALPHA; Hum a tub1; Hum a tub2; LIS3; MGC171407; MGC55332; TBA4A_HUMAN ; Testis-specific alpha-tubulin; TUBA1; TUBA1A; tuba1l; Tuba4a; Tubulin alpha 1 chain ; Tubulin alpha; Tubulin alpha-1 chain; tubulin alpha-1B chain; Tubulin alpha-4A chain ; Tubulin H2-alpha; Tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; Tubulin, alpha 4a; Tubulin, alpha, testis-specific; Tubulin, alpha-1; Alphatubulin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:1000

IF 1:250-1:500

FCM 1:1000

Immunogen

Information

Gene Name：

TUBA1B

Protein Name：

Tubulin alpha-1B chain

Gene ID：

10376 (Human)

22145 (Mouse)

316531 (Rat)

SwissPro：

P68363 (Human)

P68368 (Mouse)

Q5XIF6 (Rat)

Subcellular Location：

Cytoplasm, Cytoskeleton, Microtubule.

Immunogen：

Synthetic peptide within Human α-Tubulin. AA range: 402-451.

Specificity:

α-Tubulin Monoclonal Antibody detects endogenous levels of α-Tubulin protein.

Product images
	Fig : Western blot analysis of α-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80111, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raw264.7 cell Lane 2: Hela cell Lane 3: PC-12 cell Lane 4: NIH3T3 cell Lane 5: K562 cell Lane 6: Rat brain Lane 7: Hek293 cell Lane 8: A431 cell Lane 9: Jurkat cell Exposure time: 7 seconds Predicted band size: 50 kDa Observed band size: 52 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-blood vessel tissue with Rabbit anti-α-Tubulin (AWA80111) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80111) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.