α-tubulin Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80032

应用：

WB,IHC,IF,ELISA

反应性：

Human,Mouse,Rat,Chicken

来源：

Rabbit

规格 价格 库存
50μL ￥580 1-3个工作日
100μL ￥920 1-3个工作日
500μL ￥3800 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Chicken

Molecular Wt:

50 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:100-1:300

IF 1:200-1:1000

ELISA 1:5000

Immunogen

Information

Gene Name：

TUBA1A

Protein Name：

Tubulin alpha-1A chain

Gene ID：

7846/10376 (Human)

22142/22143 (Mouse)

64158/500929 (Rat)

SwissPro：

Q71U36/P68363 (Human)

P68369/P05213 (Mouse)

P68370/Q6P9V9 (Rat)

Subcellular Location：

Cytoplasm, Cytoskeleton.

Immunogen：

The antiserum was produced against synthesized peptide derived from human Tubulin alpha. AA range: 402-451.

Specificity:

α-tubulin Polyclonal Antibody detects endogenous levels of α-tubulin protein.

Product images
	Fig : Western blot analysis of α-tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80032, 1/16000) was used in TBST-1%TWEEN20 at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: Hela cell lysate Lane 2: HEK293 cell lysate Lane 3: PC12 cell lysate Lane 4: NRK-49F cell lysate Lane 5: HepG2 cell lysate Lane 6: Jurkat cell lysate Lane 7: RBL-2H3 cell lysate Lane 8: RAW264.7 cell lysate Lane 9: 3D4/21 cell lysate Lane 10: K562 cell lysate Predicted molecular weight: 50 kDa Observed molecular weight: 55 kDa Exposure time: 7 seconds
	Fig : Western blot analysis of α-tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80027, 1/16000) was used in (TBST-1%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate Lane 2: 4T1 cell lysate Lane 3: SHZ-88 cell lysate Lane 4: A549 cell lysate Lane 5: A431 cell lysate Predicted molecular weight: 50 kDa Observed molecular weight: 55 kDa Exposure time: 7 seconds