β-Tubulin Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80021

应用：

WB,IHC,IF,FCM,ELISA

反应性：

Human,Mouse,Rat,Monkey

来源：

Mouse

规格 价格 库存
50μL ￥580 1-3个工作日
100μL ￥920 1-3个工作日
500μL ￥3800 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

50 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Other Names:

Beta 4 tubulin; Beta 5 tubulin; TBB5_HUMAN; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; BetaTubulin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:200-1:1000

IF 1:200-1:1000

FCM 1:200-1:400

ELISA 1:10000

Immunogen Information

Gene Name：

TUBB

Protein Name：

Tubulin beta chain

Gene ID：

203068 (Human)

22154 (Mouse)

29214 (Rat)

SwissPro：

P07437 (Human)

P99024 (Mouse)

P69897 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Recombinant fragment of human TUBB1. AA range:1-444.

Specificity:

β-Tubulin Monoclonal Antibody detects endogenous levels of β-Tubulin protein.

Product images
	Fig : Western blot analysis of β-Tubulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80021, 1/5000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: NIH3T3 cell Lane 3: Raw264.7 cell Lane 4: Hela cell Lane 5: RBL-2H3 cell Lane 6: Rat brain Lane 7: SHZ-88 cell Exposure time: 15 seconds Predicted band size: 50 kDa Observed band size: 50 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Mouse anti-β-tubulin (AWA80021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of Hela cells labeling β-Tubulin with Mouse Monoclonal anti-β-Tubulin antibody (AWA80021) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse Monoclonal anti-β-Tubulin antibody (AWA80021) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-bladder tissue with Mouse anti-β-tubulin (AWA80021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Mouse anti-β-tubulin (AWA80021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-lung tissue with Mouse anti-β-tubulin (AWA80021) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80021) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.