GAPDH Recombinant Rabbit Monoclonal Antibody

货号:
AWA80016
应用:
WB,IHC-P,IF-C,IF-T,FCM
反应性:
Human,Mouse,Rat,Chicken,Zebrafish
来源:
Rabbit
  • 50μL
  • ¥580
  • 1-3个工作日
  • 100μL
  • ¥920
  • 1-3个工作日
  • 500μL
  • ¥3800
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Chicken,Zebrafish

    Molecular Wt:

    36 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; Glyceraldehyde-3-phosphate dehydrogenase


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:2000-1:20000
    IHC-P 1:100-1:5000
    IF-C 1:50-1:1000
    IF-T 1:100-1:5000
    FCM 1:1000



    Immunogen

    Information

    Gene Name:

    GAPDH

    Protein Name:

    Glyceraldehyde-3-phosphate dehydrogenase


    Gene ID:

    2597 (Human)    
    14433 (Mouse)    
    24383 (Rat)

    SwissPro:

    P04406 (Human)    
    P16858 (Mouse)    
    P04797 (Rat)


    Subcellular Location:

    Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.


    Immunogen:

    Recombinant protein within mouse GAPDH. AA range: 94-333.


    Specificity:

    GAPDH Monoclonal Antibody detects endogenous levels of GAPDH protein.




    Product images
    GAPDH Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80016, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: L929 cell
    Lane 2: SH-SY5Y cell
    Lane 3: NIH3T3 cell
    Lane 4: PC12 cell
    Lane 5: Hela cell
    Lane 6: Jurkat cell
    Lane 7: A549 cell
    Lane 8: MCF-7 cell
    Lane 9: Rat brain
    Lane 10: THP-1 cell

    Exposure time: 7 seconds
    Predicted band size: 36 kDa
    Observed band size: 36 kDa
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