GAPDH Mouse Monoclonal Antibody
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- 50μL
- ¥580
- 1-3个工作日
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- 100μL
- ¥920
- 1-3个工作日
-
- 500μL
- ¥3800
- 1-3个工作日
Product Details | Host Species: Mouse | Reactivity: Human,Mouse,Rat,Mk,Dg,Ch,Hamster,Rabbit,Pig,sheep,Insect,Yeast,Bovine | Molecular Wt: 36 kDa | |
Clonality: Monoclonal | Isotype: IgG1 | Concentration: 1 mg/ml | ||
Other Names: GAPDH; GAPD; CDABP0047; OK/SW-cl.12; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; Glyceraldehyde-3-phosphate dehydrogenase | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:2000-1:20000 | |||
Immunogen Information | Gene Name： GAPDH | Protein Name： Glyceraldehyde-3-phosphate dehydrogenase | ||
Gene ID： 2597 (Human) | SwissPro： P04406 (Human) | |||
Subcellular Location： Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. | ||||
Immunogen： Synthetic Peptide of human GAPDH. AA range: 200-300. | ||||
Specificity: GAPDH Monoclonal Antibody detects endogenous levels of GAPDH protein. | ||||
| Product images | |
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Fig: Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Mouse Monoclonal Antibody (AWA80012) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with GAPDH Mouse Monoclonal Antibody (AWA80012) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA80012, 1/20000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell Lane 2: Rat brain Lane 3: Mouse brain |
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Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80012, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: A549 cell Lane 3: NIH3T3 cell Lane 4: Jurkat cell Lane 5: MCF-7 cell Lane 6: PC-12 cell Exposure time: 15 seconds Predicted band size: 36 kDa Observed band size: 36 kDa |
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Fig: Fluorescence immunohistochemical analysis of Rat-testices tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-GAPDH antibody (AWA80012) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80012) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig: Fluorescence immunohistochemical analysis of Mouse-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-GAPDH antibody (AWA80012) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80012) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with mouse anti-GAPDH antibody (AWA80012) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA80012 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with mouse anti-GAPDH antibody (AWA80012) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA80012 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Mouse anti-GAPDH (AWA80012) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80012) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 50μL
- ¥580
- 1-3个工作日
-
- 100μL
- ¥920
- 1-3个工作日
-
- 500μL
- ¥3800
- 1-3个工作日
-
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