GAPDH Rabbit Polyclonal Antibody
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- 50μL
- ¥580
- 1-3个工作日
-
- 100μL
- ¥920
- 1-3个工作日
-
- 500μL
- ¥3800
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Rabbit,Ch,Mk,sheep,X,Fish,Chicken,Guineapig,Guineapig,Duck | Molecular Wt: 36 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: GAPDH; GAPD; CDABP0047; OK/SW-cl.12; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; Glyceraldehyde-3-phosphate dehydrogenase | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:2000-1:20000 | |||
Immunogen Information | Gene Name： GAPDH | Protein Name： Glyceraldehyde-3-phosphate dehydrogenase | ||
Gene ID： 2597 (Human) | SwissPro： P04406 (Human) | |||
Subcellular Location： Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. | ||||
Immunogen： Recombinant Protein of GAPDH. | ||||
Specificity: GAPDH Polyclonal Antibody detects endogenous levels of GAPDH protein. | ||||
| Product images | |
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Fig: Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Rabbit Polyclonal Antibody (AWA80009) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with GAPDH Rabbit Polyclonal Antibody (AWA80009) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80009, 1/5000) was used in TBST at room temperature for 2 hours. Goat Anti-rabbit IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1:Hela cell Lane 2: Rat heart Lane 3: Rat brain Lane 4: Rat liver |
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Fig: Fluorescence immunohistochemical analysis of Mouse-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GAPDH antibody (AWA80009) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80009) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-GAPDH antibody (AWA80009) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA80009) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GAPDH antibody (AWA80009) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA80009) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 50μL
- ¥580
- 1-3个工作日
-
- 100μL
- ¥920
- 1-3个工作日
-
- 500μL
- ¥3800
- 1-3个工作日
-
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