β-actin Rabbit Polyclonal Antibody

货号:
AWA80002
应用:
WB,IF,ELISA
反应性:
Human,Mouse,Rat,Chicken, Globefish,Bovine,Hamster,Pig, Ovine,Cat,Pig,Dog,Sheep
来源:
Rabbit
  • 50μL
  • ¥580
  • 1-3个工作日
  • 100μL
  • ¥920
  • 1-3个工作日
  • 500μL
  • ¥3800
  • 1-3个工作日
  • 产品概述
  • 参考文献
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Chicken,

    Globefish,Bovine,Hamster,Pig,

    Ovine,Cat,Pig,Dog,Sheep

     

    Molecular Wt:

    42 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β; Actin Beta; β-Actin; Beta-Actin; Beta actin; PS1TP5BP1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:5000

    IF 1:50-1:200

    ELISA 1:20000

     



    Immunogen

    Information

    Gene Name:

    ACTB 

    Protein Name:

    Actin, cytoplasmic 1


    Gene ID:

    60 (Human)     

    11461 (Mouse)     

    81822 (Rat)

    SwissPro:

    P60709 (Human)     

    P60710 (Mouse)  

    P60711 (Rat)

     


    Subcellular Location:

    Cytoplasm, cytoskeleton. Nucleus.


    Immunogen:

    Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1- 80.

     


    Specificity:

    β-actin Polyclonal Antibody detects endogenous levels of β-actin protein.


    Product images
    β-actin Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Beta actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80002, 1/5000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HEPG2 cell
    Lane 2: Jurkat cell
    Lane 3: RAW264.7 cell
    Lane 4: HEK-293 cell
    Lane 5: A549 cell
    Lane 6: HELA cell
    Lane 7: NIH3T3 cell
    Lane 8: HEPA1-6 cell
    β-actin Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA80002, 1/10000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: K562 cell
    Lane 3: A549 cell
    Lane 4: COS7 cell
    Lane 5: PC-12 cell
    Lane 6: MCF-7 cell
    Lane 7: Raw264.7 cell
    Lane 8: THP-1 cell


    Exposure time: 45 seconds
    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    1. Fan, Shuangshi et al. “Molecular prognostic of nine parthanatos death-related genes in glioma, particularly in COL8A1 identification.” Journal of neurochemistry vol. 168,3 (2024): 205-223. doi:10.1111/jnc.16049 .  PubMed:38225203

    2. Liu, Ying et al. “PPAR-α inhibits DHEA-induced ferroptosis in granulosa cells through upregulation of FADS2.” Biochemical and biophysical research communications vol. 715 (2024): 150005. doi:10.1016/j.bbrc.2024.150005PubMed:38678785

    3. Wang, Fan et al. “Silencing BMAL1 promotes M1/M2 polarization through the LDHA/lactate axis to promote GBM sensitivity to bevacizumab.” International immunopharmacology vol. 134 (2024): 112187. doi:10.1016/j.intimp.2024.112187. PubMed:38733825

    4. Wu, Zhifeng et al. “Silencing p75NTR regulates osteogenic differentiation and angiogenesis of BMSCs to enhance bone healing in fractured rats.” Journal of orthopaedic surgery and research vol. 19,1 192. 20 Mar. 2024, doi:10.1186/s13018-024-04653-8.  PubMed:38504358

    5. Tian, Zhenyang et al. “LncARSR promotes glioma tumor growth by mediating glycolysis through the STAT3/HK2 axis.” Cytokine vol. 180 (2024): 156663. doi:10.1016/j.cyto.2024.156663.  PubMed:38815522

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