β-actin Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA80001

应用：

WB,IF-C,IF-T,FCM,ELISA

反应性：

Human,Mouse,Rat,Pig, Hamster,Monkey,Dog

来源：

Mouse

规格 价格 库存
50μL ￥580 1-3个工作日
100μL ￥920 1-3个工作日
500μL ￥3800 1-3个工作日

产品概述

参考文献

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat,Pig,

Hamster,Monkey,Dog

Molecular Wt:

42 kDa

Clonality:

Monoclonal

Isotype:

IgG2b

Concentration:

1 mg/ml

Other Names:

ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β; Actin Beta; β-Actin; Beta-Actin; Beta actin; PS1TP5BP1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:10000-1:20000

IF-C 1:200-1:1000

IF-T 1:100-1:1000

FCM 1:200-1:400

ELISA 1:10000

Immunogen

Information

Gene Name：

ACTB

Protein Name：

Actin, cytoplasmic 1

Gene ID：

60 (Human)

11461 (Mouse)

81822 (Rat)

SwissPro：

P60709 (Human)

P60710 (Mouse)

P60711 (Rat)

Subcellular Location：

Cytoplasm, cytoskeleton. Nucleus.

Immunogen：

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin.

Specificity:

β-actin Monoclonal Antibody detects endogenous levels of β-actin protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections). with mouse anti-β-actin antibody (AWA80001) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA80001) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner
	Fig: Fluorescence immunohistochemical analysis of Rat-placenta tissue (Formalin/PFA-fixed paraffin-embedded sections). with mouse anti-β-actin antibody (AWA80001) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA80001) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80001, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: NIH/3T3 cell Lane 3: THP-1 cell Lane 4: U251 cell Lane 5: A549 cell Lane 6: C2C12 cell Lane 7: HEPG2 cell Lane 8: HCT-116 cell Lane 9: HUVEC cell Lane 10: LLC cell Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 15 seconds
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80001, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NRK-49F cell Lane 2: J774A.1 cell Lane 3: ID8 cell Lane 4: MC38 cell Lane 5: GL261 cell Lane 6: HEPA1-6 cell Lane 7: HT1080 cell Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 15 seconds
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80001, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 4T1 cell Lane 2: HT22 cell Lane 3: N-2A cell Lane 4: L929 cell Lane 5: AML12 cell Lane 6: RM-1 cell Lane 7: COS7 cell Lane 8: 3D4/21 cell Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 15 seconds
	Fig : Western blot analysis of β-actin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA80001, 1/10000) was used in PBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell Lane 2: SW620 cell Lane 3: PC-3 cell Lane 4: Ishikawa cell Lane 5: SW480 cell Lane 6: 786-O cell Lane 7: A2058 cell Lane 8: BEAS-2B cell Lane 9: RBL-2H3 cell Lane 10: SHZ-88 cell Predicted molecular weight: 42 kDa Observed molecular weight: 42 kDa Exposure time: 15 seconds
	Immunocytochemistry analysis of Hela cells labeling β-actin with mouse anti-β-actin antibody （AWA80001）at 1/200 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with mouse anti-β-actin antibody （AWA80001）at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-mouse IgG H&L (iFluor™ 594, AWS0004c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

1. Li, Xiuping et al. “Isoquercitrin alleviates OGD/R-induced oxidative stress and impaired mitochondrial biogenesis in SH-SY5Y cells via the NRF1/TFAM pathway.” Cell biochemistry and biophysics, 10.1007/s12013-024-01355-0. 18 Jun. 2024, doi:10.1007/s12013-024-01355-0. PubMed：38888870