TRAF6 Rabbit Polyclonal Antibody

货号:
AWA70797
应用:
IHC-P,IF-C,IF-T
反应性:
Human,Mouse,Rat,Chicken,Zebrafish
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,Chicken,Zebrafish

    Molecular Wt:

    60 KDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85; MGC:3310; TRAF6


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    IHC-P 1:100-1:500
    IF-C 1:50-1:200
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    TRAF6

    Protein Name:

    TNF receptor-associated factor 6


    Gene ID:

    7189 (Human)    
    22034 (Mouse)    
    311245 (Rat)

    SwissPro:

    Q9Y4K3 (Human)    
    P70196 (Mouse)    
    B5DF45 (Rat)


    Subcellular Location:

    Cytoplasm. Cytoplasm, cell cortex. Nucleus. Lipid droplet.


    Immunogen:

    Synthetic peptide within C-terminal human TRAF6.


    Specificity:

    TRAF6 Polyclonal Antibody detects endogenous levels of TRAF6 protein.




    Product images
    TRAF6 Rabbit Polyclonal Antibody - 1 Fig: Fluorescence immunohistochemical analysis of Rat-kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with rabbit anti-TRAF6 antibody (AWA70797) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA70797) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
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