Smad2/3 Rabbit Polyclonal Antibody

货号:
AWA48869
应用:
WB,IHC,IF,ELISA
反应性:
Human,Mouse,Rat,Monkey,Pig
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat,

    Monkey,Pig

     

    Molecular Wt:

    52 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC 1:100-1:300

    IF 1:50-1:200

    ELISA 1:10000

     



    Immunogen

    Information

    Gene Name:

    SMAD2/SMAD3

    Protein Name:

    Mothers against decapentaplegic homolog 2/3


    Gene ID:

    4087/4088 (Human)     

    17126/17127 (Mouse)     

    29357/25631 (Rat)

     

    SwissPro:

    Q15796/P84022 (Human)     

    Q62432/Q8BUN5 (Mouse)     

    O70436/P84025 (Rat)


    Subcellular Location:

    Cytoplasm. Nucleus.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human Smad2/3. AA range: 1-50.

     


    Specificity:

    Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.


    Product images
    Smad2/3 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of SMAD2/3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA48869, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SK-Hep-1 cell
    Lane 2: PANC-1 cell

    Predicted molecular weight:52 kDa(SMAD2)/48KD(SMAD3)
    Observed molecular weight:50-60 kDa

    Exposure time:3 min
    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg