HDAC3 Recombinant Rabbit Monoclonal Antibody

货号：

AWA12693

应用：

WB,IHC-P,IF-C,IP

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 49 kDa
Observed MW: 49 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

HDAC3; HDAC 3; Histone deacetylase 3; HD3; RPD3-2; RPD3 2; RPD3-2; SMAP45

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-C 1:100-1:800
IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.

Immunogen
Information

Gene Name:

HDAC3

Protein Name:

Histone deacetylase 3

Gene ID:

8841 (Human)
15183 (Mouse)
84578 (Rat)

SwissPro:

O15379 (Human)
O88895 (Mouse)
Q6P6W3 (Rat)

Immunogen
Information

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm, cytosol.

Immunogen:

Synthetic peptide within C-terminal human HDAC3.

Specificity:

HDAC3 Monoclonal Antibody detects endogenous levels of HDAC3 protein.

Product images
	Fig : Western blot analysis of HDAC3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12693, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: HEK293 cell Lane 3: K562 cell Lane 4: HepG2 cell Lane 5: NIH/3T3 cell Lane 6: HEPA1-6 cell Lane 7: Mouse brain Lane 8: Mouse testicular Lane 9: Rat testicular Predicted molecular weight:49 kDa Observed molecular weight:49 kDa

	Fig : Western blot analysis of HDAC3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12693, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL60 cell Lane 2: Raji cell Lane 3: RAW 264.7 cell Lane 4: PC12 cell Lane 5: HSC-T6 cell Lane 6: Rat brain Predicted molecular weight:49 kDa Observed molecular weight:49 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-HDAC3 antibody (AWA12693) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12693) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testicle tissue with Rabbit anti-HDAC3 antibody (AWA12693) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12693) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of Hela cells labeling HDAC3 with Rabbit anti-HDAC3 antibody (AWA12693) at 1/200 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-HDAC3 antibody (AWA12693) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-HDAC3 antibody (AWA12693) at 1/3000 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12693) at 1/3000 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.