Ubiquitin Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12690

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

26 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Polyubiquitin B; UBB; ubiquitin; Ubiquitin; ubiquitin B; Ubiquitin B; FLJ25987; MGC8385; RPS 27A; RPS27A; UBA 52; UBA 80; UBA52; UBA80; UBB_HUMAN; UBC; UBCEP 1; UBCEP 2; UBCEP1; UBCEP2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IHC-P 1:200-1:10000

IF-C 1:50-1:500

IF-T 1:50-1:1000

FCM 1:1000

Immunogen

Information

Gene Name：

UBB

Protein Name：

Polyubiquitin-B

Gene ID：

7314/7316/6233/7311 (Human)

22187 (Mouse)

192255 (Rat)

SwissPro：

P0CG47/P0CG48/P62979/P62987 (Human)

P0CG49/P62991 (Mouse)

P0CG51/P62989 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Mitochondrion outer membrane.

Immunogen：

Synthetic peptide within human Ubiquitin. AA range: 1-50.

Specificity:

Ubiquitin Monoclonal Antibody detects endogenous levels of Ubiquitin protein.

Product images
	Fig: Immunocytochemistry analysis of Hela cells labeling Ubiquitin with Rabbit anti-Ubiquitin antibody (AWA12690) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ubiquitin antibody (AWA126902) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Ubiquitin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12690, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: N2A cell lysate Lane 4: HEK293 cell lysate Lane 5: PC12 cell lysate Lane 6: HepG2 cell lysate Lane 7: HSC-T6 cell lysate Lane 8: K562 cell lysate Lane 9: L929 cell lysate Lane 10: A549 cell lysate Predicted molecular weight: 26KD Observed molecular weight: 26KD Exposure time: 15 seconds
	Fig : Western blot analysis of Ubiquitin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12690, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.(10% SDS-PAGE gel.) Positive control: Lane 1: MCF-7 cell lysate Lane 2: SHZ-88 cell lysate Lane 3: 4T1 cell lysate Lane 4: Jurkat cell lysate Lane 5: RAW264.7 cell lysate Lane 6: 3D4/21 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-3 cell lysate Predicted molecular weight: 26KD Observed molecular weight: 26KD Exposure time: 15 seconds
	Fig: Fluorescence immunohistochemical analysis of mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ubiquitin antibody ( AWA12690 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12690 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti-Uboquitin antibody (AWA12690) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12690) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ubiquitin antibody ( AWA12690 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12690 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of rat-liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ubiquitin antibody ( AWA12690 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12690 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.