ATG7 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12685
应用:
WB,IF-C,FCM,IP
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    78 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    APG7 LIKE; APG7L; ATG7; ATG 7; Apg 7; Autophagy related protein 7; Autophagy-related protein 7; APG7 autophagy 7 like; APG7 like; APG7-like; GSA7; hAGP7; GSA 7; GSA7; hAGP7


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    FCM 1:50-1:100
    IP 1-2ug/sample



    Immunogen

    Information

    Gene Name:

    ATG7

    Protein Name:

    Ubiquitin-like modifier-activating enzyme ATG7


    Gene ID:

    10533 (Human)    
    74244 (Mouse)    
    312647 (Rat)

    SwissPro:

    O95352 (Human)    
    Q9D906 (Mouse)    
    Q641Y5 (Rat)


    Subcellular Location:

    Cytoplasm.


    Immunogen:

    Synthetic peptide within human ATG7. AA range: 654-703.


    Specificity:

    ATG7 Monoclonal Antibody detects endogenous levels of ATG7 protein.




    Product images
    ATG7 Recombinant Rabbit Monoclonal  Antibody - 1 Fig: Immunocytochemistry analysis of Hela cells labeling ATG7 with Rabbit anti-ATG7 antibody (AWA12685) at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ATG7 antibody (AWA12685) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    ATG7 Recombinant Rabbit Monoclonal  Antibody - 2 Fig: Immunocytochemistry analysis of Hela cells labeling ATG7 with Rabbit anti-APG7 antibody (AWA12685) at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-APG7 antibody (AWA12685)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    ATG7 Recombinant Rabbit Monoclonal  Antibody - 3 Fig : Western blot analysis of ATG7 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12685, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: THP-1 cell
    Lane 2: Jurkat cell
    Lane 3: HELA cell
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